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Overview :
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| Bioprocess |
Laboratory Analysis |
On-line Analysis |
| Fermentation |
Lactate |
Ethanol |
| Process Monitoring |
Glucose |
Methanol |
| Sucrose |
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ProcessTRACE
On-line Monitoring
ProcessTRACE is a special solution provided by TRACE Analytics for on-line operations to monitor microorganism cultures.The analysis of glucose in culture media requires sampling under sterile conditions, while ensuring reproducibility and relevance of the sample. Three sampling options are available with ProcessTRACE: filtration probe, dialysis probe (patent pending) and bypass module. All systems enable reliable sterile sampling directly from the bioreactor. |
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| ProcessTRACE is primarily used for on-line monitoring of industrial or laboratory cultures. For all types of cultures (batch, fed-batch, continuous culture), continuous glucose analysis using ProcessTRACE can be carried out. Individual sampling for on-line and off line analysis is feasible. |

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ProcessTRACE consists of a housing tailored to the specific application and environmental conditions. The front panel comprises all the mechanical elements. The electronics are of modular design and allow a multiple data output.
The analysis is performed with support of a sensor unit, which consists of an electrochemical biosensor. System control and data processing are achieved via application-specific, user-friendly software. |
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Features |
| measuring range 0.01 - 40 g/l
measurement frequency up to 30 per hour
system runs automatically including calibration routine
high lifetime of the sensor (at least 14 days or 5000 measurements)
standard deviation < 3.0 % for 10 g glucose per litre |
| Principles of measurement |
| enzymatic-amperometric
flow diffusion technique for precise and reproducible
measurements
biosensor based detection system |
| Technology |
| no sample preparation
none volume based dialysis sampling
sterile sampling with dialysis, filtration and bypass module
ProcessTRACE including dialysis probe particularly for
research and development (small bioreactors)
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ProcessTRACE technical data |
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| General specifications |
| Operating modes | on-line and off line |
| Measurement principle | enzymatic-amperometric |
| Sampling method | filtration, dialysis via probe, dialysis via bypass |
| Analytes | see application notes; available are glucose (high and low level) lactate, methanol, ethanol and sucrose |
| Measurement frequency | up to 30 per hour |
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| Flowcell |
| Configuration | wall-jet |
| Electrodes | sensorchips as biosensors; special sensors and enzyme reactors on request |
| Material | PEEK / PMMA |
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| External connections |
| Analogue output | 0...10V / 0...20mA / 4...20mA |
| Parallel output | Centronics |
| Serial output | RS 232 |
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| Physical specifications |
| Dimensions | (WxHxL) 470 x 160 x 400 mm |
| Weight | kg 8,7 |
| Power | 110...120 / 220...240 VAC; 50/60 Hz |
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